Coding
Part:BBa_K2926051:Design
Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
Fusion protein of Opy2 from yeast and mCherry
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To enable proper protein folding of both subdomains we designed a short glycine-serine-linker sequence inbetween them.
Source
The extracellular domain of Opy2 was generated via add-on PCR while mCherry was obtained from the iGEM part BBa_J06504